Figure 1.

Induction of dendritic cell death by silica nanoparticles. For determining cell viability of DC2.4 cells after treatment with silica nanoparticles, cells were treated with silica nanoparticles for 24 h and 48 h. Cell viability was determined using MTT assay (A) and trypan blue staining (B). Cells were treated with silica nanoparticles (40 µg/ml) for 24 h and 7-AAD (PerCP) and annexin V-FITC staining was used to analyze the DC2.4 cell death. The percentages of stained/unstained cells are shown in each quadrant (C). The total sum of annexin V-positive cells was quantified (D). Results represent the mean±SD from triplicates. ^*p<0.05, ^**p<0.01, ^***p<0.001 for one-way ANOVA.