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. 2012 Jun 30;12(3):104–112. doi: 10.4110/in.2012.12.3.104

Figure 2.

Figure 2

Silica nanoparticles affect the expression of surface antigens on dendritic cells. Bone marrow-derived DCs were generated by culturing bone marrow cells with 10 ng/ml of GM-CSF and 10 ng/ml of IL-4 for 6 days. Thereafter, cells were co-cultured with silica nanoparticles (40 µg/ml) for 24 h (A). On the other hand, bone marrow-derived cells were cultured with 10 ng/ml of GM-CSF, 10 ng/ml of IL-4, and silica nanoparticles (10 µg/ml) for 7 days (B). Cells were allowed to react with appropriate antibodies at 4℃ for 30 min for the detection of CD11c, CD54, CD80, CD86 and MHC class II. Cells were analyzed using a FACSCanto™II. The data are represented as relative Mean Fluorescence Intensity (MFI).