Figure 4.
IL10 treatment diminishes TAC-induced left ventricular fibrosis. Masson`s Trichome staining was performed to check the collagen deposition in LV tissue sections. Stained sections were analyzed with Image-Pro Plus 6.3 software. a–c). TAC significant increased interstitial and perivascular fibrosis. TAC-induced increased fibrosis was attenuated by IL10 treatment. OM=20X. d & e). Matrix metalloproteinase activity was measured in whole tissue lysates by gelatin zymography. A significant increase in MMP2 activity was observed in constricted animals. IL10 treatment largely abolished the TAC induced MMP2 activity. ***p<0.001 vs. sham, **p<0.01 vs. sham, ##p<0.01 vs. WT-TAC (N=4–5).