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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Circulation. 2012 Jun 15;126(4):418–429. doi: 10.1161/CIRCULATIONAHA.112.112185

Figure 6.

Figure 6

IL10 treatment reduces TAC-induced apoptotic cell death and caspase3 activation in mice at day 28-post constriction. Formalin fixed and paraffin embedded sections were stained with TUNEL reagents (green) and α-SA (Texas-red). Nuclei were counter stained with DAPI. TUNEL positive cells (bright green) were visualized by fluorescence microscope at 20X magnification. a & b). No cell death were observed in WT-sham mice. Transverse aortic constriction significantly increased cell death in mice. IL10 significantly reduced TAC-induced cell death. c). Whole tissue lysates were prepared from left ventricle and cleaved caspase3 and Bcl2 levels were measured. Increase caspase3 cleavage were found in WT-TAC animals, however, IL10 treatment markedly reduced it. In contrast, we did not see any significant difference on Bcl2 level after TAC surgery or with IL10 treatment. ***p<0.001 vs. WT-sham, *p<0.05 vs. WT-sham, ##p<0.01 vs. WT-TAC (N=4).