Fig. 5.

YM201636 (YM) at 800 nM, but not at 160 nM, triggers aberrant vacuolation phenotype. CHO-T (a and b), 3T3L1 fibroblasts (c and d), differentiated 3T3L1 adipocytes (e and f), and HEK293 DB4 cells (g and h) were treated with YM201636 at the indicated concentrations for 40 min and fixed. Images were obtained by Hoffman modulation. Pronounced vacuoles are seen only at 800 nM but not at 160 nM of YM201636. In 3T3L1 adipocytes aberrant vacuolation morphology is not apparent at either concentration. Scale bar, 10 μm.