Figure 3.

The expression of osteoglycin in the amygdala was analyzed using immunohistochemistry (IHC) in the three groups; CTL-MI, STR-HI, and STR-LI. The representative IHC result shows specific decreased expression of osteoglycin in the central (CeA), basolateral (BLA), and medial (MeA) nuclei of the amygdala of STR-HI mice (A). The fluorescence signal density of each nucleus was quantified using the Image J program (NIH), (B) and the strength of IHC was evaluated with one-way ANOVA and Tukey’s multiple-comparison test among groups. All tested nuclei showed significant differences in signal intensity among groups (F values of CeA, BLA, and MeA were 8.79, 14.70, and 13.55, respectively). As a result, only STR-HI mice showed significant signal decreases in each nucleus. Data are the means ± SEM (n = 3). a, P < 0.05 between CTL-MI and STR-HI mice; c, P < 0.05 between STR-HI and STR-LI mice. Scale bars are 200 μm