Figure 4.

The expression of fibromodulin in the amygdala was analyzed using IHC in the three groups; CTL-MI, STR-HI, and STR-LI mice. The results of IHC showed specific decreased expression of fibromodulin in the CeA, BLA, and MeA nuclei of the amygdala in STR-HI mice (A). The fluorescence signal density of each nucleus was quantified using the Image J program (NIH), (B) and the strength of IHC was evaluated with one-way ANOVA and Tukey’s multiple comparison test among groups. All tested nuclei showed significant differences of signal intensity among groups (F values of CeA, BLA, and MeA are 26.07, 8.915, and 10.98, respectively). As a result, only the STR-HI group showed significant signal decreases in each nucleus. Data are the means ± SEM (n = 3). a, P < 0.05 between CTL-MI and STR-HI; c, P < 0.05 between STR-HI and STR-LI mice. Scale bars are 200 μm