Table 4.
Expression of hA3B in 293 cells did not alter infectivity of XMRV or MX viruses
| Virus | Recipient cells |
Trial 1 |
Trial 2 |
Trial 3 |
Trial 4 |
Ave ± Std |
|
|---|---|---|---|---|---|---|---|
| IU/ml* | IU/ml | IU/ml | IU/ml | IU/ml | Relative infectivity** | ||
| XMRV |
293/pcDNA3 |
2.5 X 106 |
2.9 X 106 |
1.7 X 106 |
2.9 X 106 |
2.5 ± 0.52 X 106 |
1 ± 0 |
| |
293/hA3B |
2.2 X 106 |
2.3 X 106 |
2.5 X 106 |
4.3 X 106 |
2.8 ± 0.97 X 106 |
1.17 ± 0.365 |
| MX |
293/pcDNA3 |
6.5 X 106 |
6.9 X 106 |
5.1 X 106 |
8.3 X 106 |
6.7 ± 1.3 X 106 |
2.73 ± 0.231 |
| 293/hA3B | 6.9 X 106 | 9.0 X 106 | 3.6 X 106 | 8.2 X 106 | 6.9 ± 2.4 X 106 | 2.72 ± 0.462 | |
The 293 cells transfected with the plasmid encoding HA-tagged hA3B plasmids (293/hA3B) or control plasmids (293/pcDNA3) were selected by G418 and were used as recipient cells for infectivity assay. The recipient cells were infected with XMRV or MX viruses that were produced from the infected 293 T cells. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the 293/pcDNA3 infected with XMRV viruses was shown.