Fig. 4.

IK1 current density does not recover in the presence of NEM or dynasore. A and B: cell membrane capacitance (top) and whole cell IK1 current density (bottom) plotted against time of recording from cells dialyzed with NEM (1 mM; A) or with bath application of dynasore (50 μM; B). C: normalized changes in membrane capacitance comparing membrane capacitance from the last 3 min to the first 3 min of recording. D: normalized averages of IK1 current density recovery from tram-34 block in the presence of NEM or dynasore. Horizontal bars denote the presence and washout of tram-34 from the bath. Numbers on the plots correspond to the representative current density traces shown in the inset. Dash lines on the bar graphs show 100% (control). *Statistical significance (P < 0.05, paired t-test).