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Dynamin-mediated membrane internalization is Ca2+ dependent. Cell membrane capacitance plotted against time of recording from cells not dialyzed (control, top) or dialyzed with 5 mM BAPTA (bottom). Time 0 indicates whole cell formation; 50 μM dynasore were bath-treated at 5 min, followed by 1 μM Ach at 15 min. *Statistical significance (P < 0.05, paired t-test) compared with recordings before application of dynasore or Ach.
