Fig. 3.

Effect of 1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34) on FSK-induced I_sc and active K⁺ secretion in rat distal colon. A: time course of I_sc in the absence and in the presence of FSK and VO₄. VO₄ (1 mM) was added to mucosal bath, whereas 10 μM FSK was added to serosal bath (FSK/VO₄). I_sc in the presence of FSK/VO₄ was also measured in presence of 50 μM mucosal [TRAM-34 (m)] and 500 nM serosal [TRAM-34 (s)] Arrows indicate the time of addition of respective drug. B: m-s, s-m, and net K⁺ fluxes were measured in the presence of FSK/VO₄ (white bars). The K⁺ fluxes were also measured in the presence of mucosal [TRAM-34 (m); gray bars] and serosal [TRAM-34 (m/s); black bars] TRAM-34. Both I_sc and K⁺ fluxes were measured under voltage-clamp conditions.