Table 2.

Western blot detection of connexins and PACR₁

Antibody	Male:Female Gene Expression
Cx36	0.59 ± 0.03:1*
Cx43	0.46 ± 0.05:1*
PACR1	2.43 ± 1.05:1

Values are means ± SE. Western blot detection of Cx36, Cx43, and pituitary adenylate cyclase-activating peptide (PACAP) receptor (PACR₁) protein expression. β-Actin or β-tubulin normalized intensities from 3 experiments were pooled by gender and antibody type. Gene expression ratio (male:female) taken from normalized intensity values is reported.

^*Statistical significance was determined by unpaired Student's t-test with P value <0.001.