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Insulin effect on hSGLT2 wild-type and hSGLT2 S524A mutant. d-[α-methyl-14C]glucopyranoside uptake measured in control condition (open bars) and after preincubation for 2 h in DMEM supplemented with 100 pM (black bars) or 400 pM insulin (hatched bars) at 37°C from hSGLT2, hSGLT2 S624A, and hSGLT1-transfected cells. n ≥ 8 wells from at least 2 different experiments. Values are means ± SE. ***P ≤ 0.001 *P ≤ 0.05.
