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Chiral analysis of MrTPS enzyme products. The enzymes MrTPS1/MrTPS2 (A), MrTPS3 (B) and MrTPS5 (C) were expressed in E. coli, extracted, partially purified, and incubated with the substrate FPP. Products were analyzed by GC-MS with a chiral column. Retention times and spectra were compared to those of the pure standards (−)-(E)-β-caryophyllene, (−)-β-elemene and the standard mixtures (±)-(E)-β-caryophyllene and (±)-germacrene D.
