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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Dev Dyn. 2012 Feb 1;241(3):545–552. doi: 10.1002/dvdy.23747

Figure 1. The experimental setup for early electroporations.

Figure 1

(A) Electrode assembly. Electrodes were made from platinum wire threaded through the hollow plastic sleeve of a cotton swab (panels 1, 2). The wire was fixed to the top of the plastic sleeve with tacky wax (white triangle, panel 3). The bottom end of the wire was wrapped around a “female” socket pin (box, Panel 3). Panel 4 shows a magnified view of the female socket pin, (boxed area in panel 3). A “male” gold pin (panel 5) soldered to the connecting cables (not shown) is inserted into the female pin to complete the circuit. (B-E) Top-down view of HH5 embryo demonstrating the electroporation procedure. (B) India ink injection for visualizing the embryo. (C) Injection of the DNA and Fast Green solution between the vitelline membrane and the region (e.g. presumptive midbrain) of the embryo targeted for electroporation. (D-F) Electrode positions for dorsal (D) ventral (E, F) electroporations. A cross-sectional view of E is shown in F. The paler segments of the capillary tube (C) or the positive (+) electrode (D, E) depict the segments of each that lie subjacent to the vitelline membrane and are thus partially obscured from view.