(A, B) Broad (A) and focal (B) ventral EGFP expression in HH7 midbrains electroporated at HH5. All sections (A-H) are counterstained with DAPI (blue). (C, D) E4 brains showing targeted dorsolateral (C) and ventral (D) RFP electroporations. (E-H) Un-electroporated (E, G) and early-electroporated (F, H) midbrains showing that broad, unilateral EGFP electroporations do not affect FOXA2 and LMX1A/B expression in the floor plate (FP) and roof plate (RP) at E3.