Table 1.
A list of critical equipment and catalog numbers required for E1 electroporations. More detailed information is available upon request.
| Reagent | Company | Catalog # |
|---|---|---|
| Pelikan drawing ink A, black | Pelikan (Hannover, Germany) | 201665 |
| PV830 Pneumatic Picopump | World Precision Instruments, Inc. (Sarasota, FL) |
SYS-PV830 |
| Microcapillary tube size 1-5μL | Sigma-Aldrich (Milwaukee, WI) | P0549 |
| Fast Green FCF | Sigma-Aldrich (Milwaukee) | F7258 |
| Connectors, male, pin, 18AWG |
Newark Electronics (Palatine, IL) | 96F7924 |
| Connectors, female, socket, 18AWG |
Newark Electronics (Palatine, IL) | 92F5795 |
| Platinum wire, .001” bare, 10ft | A-M Systems, Inc. (Sequim, WA) | 764500 |
| Platinum wire, .002” bare, 10ft | A-M Systems, Inc. (Sequim, WA) | 765000 |
| ECM830 Electro square porator |
BTX (Holliston, MA) via VWR | 47745-928 |
| Connector cable, banana plug to micrograbber |
BTX (Holliston, MA) via VWR | 82019-192 |
| CUY21 in vivo electroporator | Bex Co. Ltd. (Tokyo, Japan) | CUY21EX |
| Transparent polyethylene tape | 3M (Austin, TX) | 70-0060-3404-8 |