Fig. 7.

IEC-6 cell proliferation in response to incubation with HA and IGF-I. IEC-6 cells were grown in DMEM supplemented with 10% FCS, penicillin (50 μg/ml), streptomycin (50 μg/ml), and ITS [insulin (5 μg/ml), transferrin (5 μg/ml), selenium (5 ng/ml)]. For all experiments, cells were switched to DMEM supplemented with 0.05% FCS, 0.1% BSA, and 5 μg/ml transferrin, along with HA and/or IGF-I, and incubated for 24 h. Proliferation was assayed by 3-(4,5-dimethlythiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT). Values are means ± SE for 3 experiments. *P < 0.0001 vs. control.