Fig. 10.

Expression of TMEM16A and bestrophin-1 mRNA in mpkCCD_c14 cells. Photograph of gel showing PCR amplification products in mouse brain and kidney tissue (positive controls) and in mpkCCD_c14 cells (mpk) after reverse transcription of mouse bestrophin-1, TMEM16A, or actin mRNA. Samples containing reverse-transcriptase (RT; +) or negative controls lacking RT (−) are included for each tissue/primer combination. *500 bp Indicates a marker of 500 bp. The specificity of all primer sets was checked by sequencing of resultant PCR products.