Fig. 8.
BAPTA-AM inhibits ATP-inducible clamp current in mpkCCDc14 cells. A: superimposed Iclamp traces of cell monolayers treated with vehicle control (solid trace) or BAPTA-AM (dashed trace). BAPTA indicates addition of BAPTA-AM (5 × 10−5) to both sides; amiloride indicates addition of amiloride (10−5 M) to the apical side; Vte indicates clamp of transepithelial voltage to −50 mV; ATP indicates addition of ATP (10−6 M) to the apical side. B: quantitation of Iclamp values in response to apical addition of ATP in control filters or in filters pretreated with BAPTA-AM (BAPTA) to the apical side (n = 12 filters). *P < 0.05.