Fig. 9.
Suramin inhibits ATP-inducible clamp current in mpkCCDc14 cells. A: superimposed Iclamp traces of cell monolayers treated with vehicle control (solid trace) or the broad spectrum P2Y receptor blocker suramin (dashed trace). Suramin indicates addition of suramin (5 × 10−4 M) to both sides; amiloride indicates addition of amiloride (10−5 M) to the apical side; Vte indicates clamp of transepithelial voltage to −50 mV; ATP indicates addition of ATP (10−6 M) to apical side. B: quantitation of Iclamp values in response to apical addition of ATP in control filters or in filters pretreated with suramin to the apical side (n = 12 filters each). *P < 0.05.