Table 1.
Target Gene | 5′-Primer | TaqMan Probe | 3′-Primer |
---|---|---|---|
β2MG | GATACATACGCCTGCAGAGTTAA | GATACATACGCCTGCAGAGTTAA CATGTG | ATGAATCTTCAGAGCATCATGAT |
Procollagen α1(1) | TCCGGCTCCTGCTCCTCTTA | TTCTTGGCCATGCGTCAGGAGGG | GTATGCAGCTGACTTCAGGGATGT |
TGF-β1 | AGAGGTCACCCGCGTGCTAA | ACCGCAACAACGCCATCTATGAGAAAACCA | TCCCGAATGTCTGACGTATTGA |
TGF-β2 | GTCCAGCCGGCGGAA | CGCTTTGGATGCTGCCTACTGCTTTAGAAAT | GCGAAGGCAGCAATTATCCT |
MMP-2 | CCGAGGACTATGACCGGGATAA | TCTGCCCCGAGACCGCTATGTCCA | CTTGTTGCCCAGGAAAGTGAAG |
TIMP-1 | TCCTCTTGTTGCTATCACTGATAGCTT | TTCTGCAACTCGGACCTGGTCATAAGG | CGCTGGTATAAGGTGGTCTCGTT |
TNF-α | GGGCCACCACGCTCTTC | ATGAGAAGTTCCCAAATGGCCTCCCTC | GGTCTGGGCCATAGAACTGATG |
β2MG, β2 microglobulin; TGF-β, transforming growth factor-β; MMP-2, matrix metalloproteinase-2; TIMP-I, tissue inhibitor of matrix metaloproteinase-1; TNF-α, tumor necrosis factor-α.