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. 2012 Jun 7;303(4):G474–G481. doi: 10.1152/ajpgi.00055.2012

Table 1.

Primer sequences for quantitative RT-PCR

Target Gene 5′-Primer TaqMan Probe 3′-Primer
β2MG GATACATACGCCTGCAGAGTTAA GATACATACGCCTGCAGAGTTAA CATGTG ATGAATCTTCAGAGCATCATGAT
Procollagen α1(1) TCCGGCTCCTGCTCCTCTTA TTCTTGGCCATGCGTCAGGAGGG GTATGCAGCTGACTTCAGGGATGT
TGF-β1 AGAGGTCACCCGCGTGCTAA ACCGCAACAACGCCATCTATGAGAAAACCA TCCCGAATGTCTGACGTATTGA
TGF-β2 GTCCAGCCGGCGGAA CGCTTTGGATGCTGCCTACTGCTTTAGAAAT GCGAAGGCAGCAATTATCCT
MMP-2 CCGAGGACTATGACCGGGATAA TCTGCCCCGAGACCGCTATGTCCA CTTGTTGCCCAGGAAAGTGAAG
TIMP-1 TCCTCTTGTTGCTATCACTGATAGCTT TTCTGCAACTCGGACCTGGTCATAAGG CGCTGGTATAAGGTGGTCTCGTT
TNF-α GGGCCACCACGCTCTTC ATGAGAAGTTCCCAAATGGCCTCCCTC GGTCTGGGCCATAGAACTGATG

β2MG, β2 microglobulin; TGF-β, transforming growth factor-β; MMP-2, matrix metalloproteinase-2; TIMP-I, tissue inhibitor of matrix metaloproteinase-1; TNF-α, tumor necrosis factor-α.