Figure 5.

Internalization of T22-GFP-H6 in SW1417 cells. (A) Isosurface representation of T22-GFP-H6-exposed SW1417 cells within a three-dimensional volumetric x-y-z data field. (B) The particulate nature of the protein and the perinuclear accumulation are clearly observed. (C) Isosurface representation of GFP-H6-exposed SW1417 cells showing lack of fluorescence. (D) MTT analysis of SW1417 cells exposed to different concentrations of T22-GFP-H6. As a control, we used determined viability of cells exposed to the storing buffer alone. Values are referred to cell viability of cultures not exposed to the buffer. (E) Dose-response curve of T22-GFP-H6 internalization in HeLa and SW1417 cells. Data adjusted to hyperbolic equations with r² = 0.9620 for HeLa cells and r² = 0.9978 for SW1417 cells (both P < 0.001). (F) Inhibition of T22-GFP-H6 internalization in HeLa and SW1417 by increasing competitor/protein ratios of the natural CXCR4 ligand SDF1α.

Notes: GFP-H6 and human GLA were included as negative controls. Asterisks indicate significant differences when comparing with any of the negative controls (P < 0.001).

Abbreviations: GFP, green fluorescent protein; GLA, α-galactosidase.