Fig. 2.

In vivo hemodynamic response to isoproterenol infusion (A). Akita^ins2 displays little response to isoproterenol (ISO) as indicated by the lack of change in end-systolic PV relationship (ESPVR) and dp/dt_max post-ISO infusion (B). Representative Western blots analysis with histograms showing relative expression of the β₁-adrenergic receptor (β₁-AR), β₂-AR and G_s (normalized to GAPDH) in the Akita^ins2 mouse and WT to determine the integrity of the β-AR signaling system (C). Data shown are means ± SD from 3 independent experiments. **P < 0.01.