Fig. 5.
CXCL12/CXCR4 expression in the Akitains2 mouse. Cryosections of left ventricular tissue were immunostained for CXCL12 (green), sarcomeric actinin (red), and DAPI (blue). CXCL12 expression is enhanced on the sarcomeric Z-lines of the cardiac myocyte in the Akitains2 myocardium (A). Representative Western blots analysis with quantitative densitometry indicate increased protein expression of CXCL12 and CXCR4 in the Akitains2 heart (B). Data shown are means ± SD; *P < 0.05. Hematoxylin and eosin staining of the left ventricle in Akitains2 and WT control show no significant inflammatory infiltrate in the myocardium (C). D: glucose induces expression of CXCL12/CXCR4 in isolated adult rat cardiomyocytes treated with either normal culture media containing 5.5 mM glucose or media supplemented with 25 mM glucose final concentration for 12 and 24 h. Total RNA was isolated and quantitative PCR of CXCL12 and CXCR4 mRNA were determined (n = 3 independent experiments; *P < 0.05; **P < 0.01). rRNA 28S was used as a control.