Fig. 2.

Pharmacological inhibition of GSK3β protects mice hearts upon I/R. Determination of myocardial ischemic injury and function with and without GSK3β inhibitor treatment as shown by left ventricular developed pressure (LVDP) recovery (A) and lactate dehydrogenase (LDH) release (B). Western blot analysis for p-GSK3β-Ser⁹ (C), p-GSK3β-Tyr²¹⁶ (D), and cytochrome c (E) in WT and ARTg hearts perfused with and without GSK3β inhibitors SB-216763 and LiCl. Treatment with SB-216763 and LiCl protected WT and ARTg mice hearts, significantly increased p-GSK3β inhibition (P < 0.05), and decreased apoptosis (n = 4–16 mice/group).