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Protein kinase C (PKC) α/β inhibition. Western blot analysis for p-Akt (A) and p-GSK3β (B). C: determination of cardiomyocyte injury as shown by LDH release. Treatment with Gö-6976 resulted in decreased cellular injury and an increase in p-GSK3β. Cells were incubated with Gö-6976 (1 μM) or its vehicle control DMSO for 1 h, followed by 30 min of hypoxia and 1 h reoxygenation (H/R).
