Fig. 5.

Ribbon diagrams comparing contacts between Arp2 and Arp3 with subunits in the mother filament in the branch junction. Top, initial EM model. Bottom, final structure at the end of the MD simulation. Arp2 is black, Arp3 is orange, mother filament subunit M6 is pink and other mother filament subunits are grey, except for important structural features that are labeled and colored red or lime. Arp2 and Arp3 residues in contact with mother filament residues are shown in lime and mother filament residues in contact with Arp2 or Arp3 are shown in red. Subunit M8 is not shown in the final snapshot as it does not form any contacts with Arp2 or Arp3. These snapshots are based on the Branch08 simulation as described in the main text.