Panel A- NRVMCs were infected with AdV-Con or AdV-ATF6. Twenty-four hours later, cultures were analyzed for ATF6 and GAPDH mRNAs. Values are mean ATF6/GAPDH mRNA ± SE, n=3 cultures per treatment.
Panel B- NRVMCs were infected with AdV-Con or AdV-ATF6. Forty-eight hours later, culture extracts were analyzed for PDIA6 and GAPDH mRNA. Values are mean PDIA6/GAPDH mRNA ± SE, n=3 cultures per treatment.
Panel C- NRVMCs were treated as described for Panel A, except extracts were analyzed for PDIA6 and GAPDH protein by immunoblotting. Relative blot intensities of PDIA6/GAPDH expressed as fold of control (bottom). Values are mean relative PDIA6/GAPDH ± SE, n=3 cultures per treatment.