Table 3.
List of genes from gene arrays, down-regulated by CBD (by more than 50%)
| Fold change and percentage of reduction | ||||
|---|---|---|---|---|
| Gene name | Description | Accession number | CBD | THC |
| Inflammatory chemokines and receptors | ||||
| Ccl2 | CCL2 | NM_011333.1 | −3.6 (72%) | −1.0 (0%) |
| Ccl7 | CCL7 | NM_013654 | −3.5 (71.4%) | 1.2 |
| Cxcl14 | CXCL14 | NM_019568 | −2.6 (61.5%) | −1.4 (29%) |
| Cx3cr1 | CX3CR1 | NM_009987.2 | −2.1 (52.4%) | −1.3 (23%) |
| Ccl6 | CCL6 | NM_009139.1 | −2.1 (52.4%) | −1.0 (0%) |
| Ccl9 | CCL 9 | NM_011338 | −2.0 (50%) | −1.2 (17%) |
| Membrane transport and secretion | ||||
| Kctd12 | Potassium channel tetramerization domain containing 12 | NM_177715.2 | −2.5 (60%) | −1.0 (0%) |
| Slc25a22 | Solute carrier family 25 (mitochondrial carrier: glutamate), member 22 | NM_026646.1 | −2.1 (52.4%) | −1.0 (0%) |
| Slc39a10 | Solute carrier family 39 (zinc transporter), member 10 | NM_172653.2 | −2.1 (52.4%) | −1.2 (17%) |
| Slc5a6 | Solute carrier family 5 (sodium-dependent vitamin transporter), member 6 | NM_177870.2 | −2.1 (52.4%) | −1.1 (9%) |
| Metabolic | ||||
| Usp2 | Ubiquitin specific protease 2 | NM_198091.1 | −2.3 (57%) | −1.3 (23%) |
| Siat7d | Sialyltransferase 7 | NM_011373.1 | −2.2 (55%) | −1.2 (17%) |
| Chst10 | Carbohydrate sulphotransferase 10 | NM_145142.1 | −2.2 (55%) | −1.8 (44%) |
| Mat2a | Methionine adenosyltransferase II, α2 | NM_145569 | −2.0 (50%) | −1.3 (23%) |
| Cell cycle and proliferation | ||||
| Ccnd1 | Cyclin D1 | NM_007631.1 | −2.9 (65.5%) | −1.2 (17%) |
| Ccne1 | Cyclin E1 | NM_007633.1 | −2.7 (63%) | −1.2 (17%) |
| Idb1 | Inhibitor of DNA binding 1 | NM_010495.1 | −2.2 (54.5%) | −1.5 (33%) |
| Cdc6 | Cell division cycle 6 homologue (S. cerevisiae) | NM_011799.1 | −2.1 (52.4%) | −1.0 (0%) |
| Ccne2 | Cyclin E2 | NM_009830.1 | −2.0 (50%) | −1.2 (17%) |
| Cdc25a | Cell division cycle 25 homologue A (S. cerevisiae) | NM_007658 | −2.0 (50%) | −1.0 (0%) |
| Host defence and adaptive response | ||||
| C1qa | Complement component 1, q subcomponent, α polypeptide | NM_007572 | −3.3 (70%) | −1.3 (23%) |
| Ebi2 | EPSTEIN-Barr virus induced gene 2 | NM_183031.1 | −2.4 (58%) | −1.3 (23%) |
| Igfbp4 | Insulin-like growth factor binding protein 4 | NM_010517.2 | −2.4 (58%) | −1.3 (23%) |
| Tlr13 | Toll-like receptor 13 | NM_205820.1 | −2.3 (56.5%) | −1.3 (23%) |
| Ly86 | Lymphocyte antigen 86 (Ly86), mRNA. | NM_010745.1 | −2.0 (50%) | −1.7 (41%) |
| Dok2 | Docking protein 2 (Dok2), mRNA. | NM_010071.1 | −2.0 (50%) | −1.1 (9%) |
| Regulation of transcription | ||||
| Zfp472 | Zinc finger protein 472 | NM_153063.3 | −5.1 (80.4%) | 1.2 |
| Fos | FBJ osteosarcoma oncogene | NM_010234.2 | −2.5 (60%) | −1.2 (17%) |
| Mef2c | Myocyte enhancer factor 2C | NM_025282.3 | −2.1 (52.4) | −1.2 (17%) |
| Tle1 | Transducin-like enhancer of split 1, homologue of Drosophila E | NM_011599.2 | −2.0 (50%) | −1.3 (23%) |
| Ase1 | CD3E antigen, ε polypeptide associated protein | NM_145822.1 | −2.0 (50%) | −1.2 (17%) |
| Regulation of translation | ||||
| Eif5a | Eukaryotic translation initiation factor 5A | NM_181582.4 | −2.6 (61.5%) | −1.2 (17%) |
| Adhesion and migration | ||||
| Pscdbp | Pleckstrin homology, Sec7 and coiled-coil domains, binding protein | NM_139200.2 | −2.1 (52.4%) | −1.7 (41%) |
| Motility and morphogenesis | ||||
| Rab3il1 | RAB3A interacting protein (rabin3)-like 1 | NM_144538.1 | −2.1 (52.4%) | −1.3 (23%) |
| Rin2 | Ras and Rab interactor 2 | NM_028724.2 | −2.0 (50%) | −1.1 (9%) |
| Unidentified | ||||
| 4921501M20Rik | RIKEN cDNA 4921501M20 gene (4921501M20Rik), mRNA. | NM_028728.1 | −2.7 (63%) | −1.2 (17%) |
| 0910001A06Rik | RIKEN cDNA 0910001A06 gene (0910001A06Rik), mRNA. | NM_144846.2 | −2.1 (52.4%) | −1.1 (9%) |
| C85492 | expressed sequence C85492 (C85492), mRNA. | NM_153540.2 | −2.1 (52.4%) | −1.0 (0%) |
List of genes down-regulated at least twofold by CBD in resting BV-2 microglial cells. Fold decrease for each gene was calculated according to the formula: Fold change = (−1) × 2−(signal log ratio)