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. 2012 Apr;165(8):2561–2574. doi: 10.1111/j.1476-5381.2011.01503.x

Figure 5.

Figure 5

(A) The inhibitory effect of AM630 on [35S]-GTPγS binding to membranes obtained from CHO cells transfected with hCB2 receptors that had or had not been pre-incubated with 10 µM AM630 for 24 h (n= 8). Mean Emax values for this inhibition with 95% confidence limits shown in parentheses are −41.7% (−36.8% and −46.7%) in untreated cells and −51.3% (−44.9% and −57.6%) in pre-incubated cells. The corresponding EC50 values of AM630 are 376 nM (210 and 674 nM) and 335 nM (175 and 641 nM), respectively. (B) The effect of 25 µM AM630 on the mean log concentration–response curve of CP55940 for stimulation of [35S]-GTPγS binding to membranes obtained from CHO cells transfected with hCB2 receptors that had been pre-incubated with 10 µM AM630 for 24 h (n= 8). In both panels, symbols represent mean values ± SEM and asterisks indicate values that are significantly less than zero (*P < 0.05; ***P < 0.001; one-sample t-test).