Table 1.

Mean K_d and B_max values of [³H]-CP55940 for its binding to specific sites determined in saturation binding experiments performed with hCB₂ CHO cells or membranes prepared from these cells

	Kd± SEM (nM)	Bmax± SEM (pmol mg−1)	Kd± SEM (%)1	Bmax± SEM (%)1	n
Untreated membranes	5.19 ± 1.45	52.6 ± 7.25	100 ± 27.9	100 ± 13.8	5
Pre-incubated membranes	7.65 ± 2.28*	96.1 ± 15.7*	147 ± 43.9	183 ± 29.8	4
Untreated cells	3.30 ± 0.85	44.4 ± 4.70	100 ± 25.8	100 ± 10.6	6
Pre-incubated cells	4.88 ± 0.99†	59.8 ± 5.70†	148 ± 30.0	135 ± 12.8	6

These experiments were performed in hCB₂ CHO cells that had been pre-incubated with 10 µM AM630 and then thoroughly washed, or in washed untreated cells, or in membranes prepared from these pre-incubated and untreated cells.

¹The mean K_d and B_max values in untreated cells or in membranes prepared from these cells have been normalized to 100% (columns 4 and 5).

Symbols denote a mean K_d or B_max value, determined in experiments with AM630-pre-incubated whole cells or with membranes obtained from these cells, that is, significantly greater than the corresponding mean K_d or B_max value determined in experiments with untreated cells or with membranes obtained from these cells (P < 0.05; *Mann–Whitney test; †Wilcoxon matched-pairs signed rank test; see Results for further information).