Table 2.

Affinity and activity data for the CB₁-GFP and CB₁(C415A)-GFP receptors in HEK-293 cells. See text for details

	CP55940 binding		GTPγS		cAMP
Receptor	Kd (nM)	Bmax (fmol·mg−1)	EC50 (nM)	Emax (% of wild-type)	EC50 (nM)	Max count (% of wild-type)
CB1-GFP	4.2 ± 0.6	174 ± 8	54 ± 2	109 ± 10	420 ± 75	98 ± 4
CB1(C415A)-GFP	3.9 ± 0.3	116 ± 12***	ND	ND	ND	2.8 ± 0.3***

Data are mean ± SEM. See text for details.

^***P < 0.001 versus CB₁-GFP receptor.

ND, not detectable.