Figure 5.

FLIPR signal representing calcium influx in GLUTag (A) and Min6 cells (B) in response to a 10-point dose titration of AR231453 (0–10 µM). GLUTag and Min6 cells were washed and loaded for 30 min at 37°C with a calcium-sensitive dye. The plate was cooled to room temperature and compounds were added while changes in intracellular calcium content were monitored for up to 20 min using a FLIPR. Maximum peak height in each well was recorded as representing calcium influx. Data are are means ± SEM (n= 8). The dose curve was generated and the EC₅₀ value was calculated with the sigmoidal dose–response (variable slope) algorithm in the GraphPad Prism 5.02 software. The data from Min6 cells did not converge.