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. 2012 Apr;165(8):2749–2757. doi: 10.1111/j.1476-5381.2011.01728.x

Figure 2.

Figure 2

S(+)AMPH induces a persistent ‘shelf’ current through the human dopamine transporter (hDAT). (A) External DA (10 µM) induced a large inward ‘peak’ current at V =−60 mV that returned to baseline when DA was removed, regardless of DA exposure time. DA peak currents were normalized to the briefest exposure time. (B) S(+)AMPH (10 µM) induced a similar inward peak current for exposures less than 30 s; however, for longer exposure times, a current that we term ‘leak’ or ‘shelf’ remained long after S(+)AMPH had been removed, and the amplitude of the shelf was proportional to the length of exposure. S(+)AMPH peak currents were normalized to the briefest exposure time. (C) Amplitude of the shelf current relative to the initial peak current plotted against exposure time of external S(+)AMPH, compared with the corresponding DA currents (n= 4, ±SEM). (D) A relatively brief and initial exposure to S(+)AMPH (20 s.), which ordinarily would not produce a shelf current, did so if the concentration of S(+)AMPH was increased from 10 to 30 µM. For the same exposure range of times and concentrations, neither DA nor S(+)AMPH induced peak or shelf currents in mock-injected oocytes (data not shown).