Figure 4.

Acute administration of Δ⁹-THC reduces male fertility. The acute effect of Δ⁹-THC on male fertility was determined by measuring embryonic (e12.5) litter sizes sired by vehicle treated CD1 wild-type (WT) males (n= 7 males with 20 litters) or CD1 wild-type males treated with 50 mg·kg⁻¹Δ⁹-THC (n= 9 males with 17 litters). The effect of vehicle (n= 5 males with 6 litters) and 50 mg·kg⁻¹Δ⁹-THC (n= 5 males with 6 litters) on litter size was also examined in mice lacking CB₁ receptors (KO). Error bars indicate SEM and P-values were calculated using two-way repeated measures anova with Bonferroni's post test (*P < 0.01).