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3D representation of the PAO1 biofilms at different oxygen saturations in FAB-GN media (minimal media supplemented with nitrate). PAO1 expresses either the fluorescent protein GFP or mRFP1. (a)-(c) 24 h old biofilms in FAB-CN media. (d)-(f) 48 h after inoculation the cells were challenged with 50 μg·ml−1 ciprofloxacin for 24 h and then stained with dead stain SYTOX® Blue. (g)-(i) Interconnected chambers of the different oxygen saturation environments.
