Figure 1.  .

Cells expressing angiogenesis markers in human limbal stroma. (A) Double immunostaining of corneo-limbo-conjunctival sections with pan cytokeratin (PCK) and vimentin (Vim) delineated the epithelium and the stroma in the limbal region. White arrow: the border between the cornea and limbus. (B) In the stroma, double immunostaining of Flk-1/CD34, CD31/CD34, CD31/VWF, and α-SMA/PDGFRβ pairs showed cells expressing potential angiogenesis markers. Although the majority of these cells were present in the perivascular location, some were found subjacent to limbal basal epithelial cells (white lines). Nuclei were counterstained by Hoechst 33342 (blue). Scale bar: 100 μm.