Figure 4.  .

Serial passages on coated Matrigel in MESCM. Single cells from collagenase-isolated clusters (Coll), D/C, or limbal RSC were seeded at a density of 1 × 10⁴ per cm² and passaged serially on coated Matrigel in MESCM, resulting in spindle cells (A) with a steady growth up to P10 and a total of more than 1 × 10¹⁰ cells (B). In contrast, limbal residual stromal cells did not grow. Compared to the expression level by cells immediately isolated (D0), spindle cells expanded from collagenase-isolated clusters and D/C exhibited a similar expression pattern up to P3, that is with more expression of vimentin, CD73, CD90, CD105, α-SMA, and PDGFRβ transcripts (C). Scale bar: 200 μm.