Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2012 Jun 25;189(3):1459–1466. doi: 10.4049/jimmunol.1200484

Figure 3.

Figure 3

A. H. polygyrus infection up-regulates Ym1, Arg1 and Fizz1 expression in peritoneal macrophages and impairs bacterial killing by macrophages. Peritoneal macrophages were isolated from normal and helminth-infected BALB/c mice at 7 days post infection. Total RNA was isolated from the cells. Ym1, Arg1 and Fizz1 expression was determined using real-time RT-PCR. Values are the fold increase compared with baseline obtained from uninfected control mice. B. H. polygyrus infection impairs bacterial killing by macrophages. Peritoneal macrophages were isolated from H. polygyrus infected mice and used for gentamicin protection assay. The results show similar numbers of viable internalized bacteria recovered in macrophages isolated from heminth-infected mice at 2 and 6 h post bacterial infection in vitro. P > 0.05. C. Immunofluorescence microscopy shows LC3-Samonella co-localization in macrophages from control and helminth-infected mice. Scale bar indicates 10 µm. The data shown are from one of three experiments with similar results.