Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2012 Jun 25;189(3):1459–1466. doi: 10.4049/jimmunol.1200484

Figure 5.

Figure 5

H. polygyrus infection reduces LC3 expression and LC3 II conversion in macrophages via a STAT6 dependent mechanism. A. Peritoneal macrophages were isolated from normal and H. polygyrus infected BALB/c mice (2 weeks after infection). Autophagy was induced in macrophages by incubating the cells overnight in the presence and absence of recombinant IFNγ (100u/ml) or by amino acid and serum starvation at 37°C for 3 h. Cell lysates were collected for detection of LC3 (LC3I and LC3II) by western-blotting. GAPDH was used as the loading control. Each lane represents the sample from an individual mouse. B and C. The bar graphs show the total LC3 density (LC3I+LC3II), LC3I, LC3II density and the LC3II/LC3I ratio (mean density + SD from 3 experiments) based on the western blotting analysis of LC3 expression (in A). D. Western blotting analysis of LC3 expression in peritoneal macrophages isolated from normal and H. polygyrus infected STAT 6 KO mice.