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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Jul 13;53(3):437–445. doi: 10.1016/j.yjmcc.2012.07.001

Table 1. KATP currents in embryonic myocytes.

KATP currents (number of cells (#) and current density), as assessed by whole cell recordings, at different developmental stages (9.5–17.5 dpc) in the WT and overexpressed STG embryonic cardiomyocytes. n represents number of embryos. N represents number of pregnancies. KATP positive cells (using cutoff value of <0.5 pA/pF as lack of KATP), demonstrated either basal (immediately after breaking in), spontaneously activating (without pinacidil exposure), or as a result of pharmacologic activation by pinacidil and inhibition by glibenclamide. Cells that demonstrated no KATP even after exposure to pinacidil for > 60 seconds, were designated KATP-negative cells. Cells that did not demonstrate KATP, but were exposed to pinacidil for less than 60 seconds were designated as undetermined (UD).

Genotype KATP
positive
cells
KATP
Negative
cells
Basal/spontaneous
KATP
# of cells
(Basal current
density, pA/pF)
Peak KATP

# of cells
(Peak current
densilty, pA/pF)
UD
cells
Total
n/N
WT 11 15 7 (1.4±0.6) 26 (11.2±5) 50 76/10
Line 4 13 6 6 (5.8±2.9) 19 (10.6±3.3) 19 38/7
Line 720 0 4 0 0 12 16/3