Table 2. Different primary antibodies used for Western blotting, ELISA and co-immunoprecipitation.
| Target protein | Clone | Primary antibody type | Supplier |
| β-actin | AC-15 | Mouse monoclonal | Sigma (#A5441) |
| CK1α | C-19 | Goat polyclonal | Santa Cruz Biotechnology (#SC-6477) |
| CK1δ | – | Rabbit polyclonal | Bethyl Laboratories (#A302-136A) |
| CK1ε | – | Mouse monoclonal | BD Transduction Laboratories (#610445) |
| MDM2† | 2A10 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| MDM2† | 4B2 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| MDM2 | 2A9 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| MDM2 | 3G5 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| MDM2 | SMP14 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| p21 | EA10 | Mouse monoclonal | Calbiochem (#OP64) |
| p53 | DO1 | Mouse monoclonal | From Borek Vojtesek, Brno, Czech Republic* |
| HP1α | 15.19s2 | Mouse monoclonal | Millipore (#05-689) |
| HSP70 | C92F3A-5 | Mouse monoclonal | Stressgen (#SPA-810) |
*
Dept. of Experimental Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
†
Unless specified otherwise, a mix of both antibodies was used for Western Blotting; 2A10 was used in ELISAs.