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. 2012 Aug 20;7(8):e43746. doi: 10.1371/journal.pone.0043746

Figure 2. TCR-like Fabs Bind Cognate pMHC with Nanomolar Affinity.

Figure 2

SPR binding response curves of fE75 binding E75/HLA-A2 (A) and ML/HLA-A2 (B) and fML binding E75/HLA-A2 (C) and ML/HLA-A2 (D) are shown. Each Fab was immobilized onto individual flow channels in an NTA-Ni chip. Kinetic data for each Fab binding each pMHC molecule were globally fit to a bimolecular reaction. Green and blue lines designate the start and end respectively of each pMHC injection. Binding curves and curve fits are drawn in black and orange respectively. Each binding curve represents a different concentration of pMHC beginning at 200 nM and decreasing to 1.5 nM in 2 fold dilutions. No binding was observed for either Fab binding non-cognate pMHC molecules up to 400 nM.