Figure 5. TCR-like Fab bind specifically to human tumor cells in SCID mice.

Mice were injected intravenously with ⁶⁴Cu-DOTA-fE75, which binds to tumors expressing both HLA-A2 and HER2/neu in vivo. Coronal views of small-animal PET images with coregistered CT images of SCID mice bearing human tumor (SKOV3 or SKOV3 HLA-A2) xenografts (A and B) in flanks at 1 h post-injection. Intensities of PET slices were scaled to the same maximum. Cropped views of only the tumors designated by the white boxes for the mice presented in A and B were shown in C and D respectively. The standardized uptake values (SUV) for ⁶⁴Cu-DOTA-fE75 in the tumors and selected organs over time were shown below each respective mouse (E and F). B  =  bladder, h  =  humeral muscle, k  =  kidneys, l  =  liver, ct  =  control HLA-A2 negative tumor; SKOV3 cell line, pt  =  HLA-A2 positive tumor; SKOV3 HLA-A2.