Table 2. Vesicle movement prior to fusion with the plasma membrane.
TOTAL | Unknowna | Fusions preceded by ∼1 µm linear displacement | Fusions in which linear displacement was undetectedb |
221 | 47 | 4 | 170 |
Vesicle movements prior to fusion with the plasma membrane were determined in 3T3-L1 adipocytes (n = 4 cells) transfected with mCherry-IRAP-pHluorin and stimulated with 100 nM insulin.
Vesicle movements were scored as unknown when high local vesicle density or low mCherry fluorescence intensity made determination of movement difficult.
Linear displacement of ∼1 µm or more was undetected prior to fusion of vesicle.