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. 2012 Aug 20;7(8):e43028. doi: 10.1371/journal.pone.0043028

Figure 4. Pairwise variation analysis among sequential normalization factors calculated by geNorm.

Figure 4

Determination of the optimal number of control genes by calculation of the pairwise variation coefficient. A value from 0.15 to 0.20 was determined as an appropriate cut-off. To the right in each group, the increasing values are due to the inclusion of unstable reference genes. Group 1: E18, P1 and P4; Group 2: P1, P4 and P10; Group 3: P1, P4, P10, P14, P45; Group 4: P10, P14 and P45; Group 5: all ages.