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. 2012 Aug 1;186(3):246–254. doi: 10.1164/rccm.201201-0134OC

Figure 8.

Figure 8.

IL-5 is protective in human sepsis. (A) Serum IL-5 was measured by ELISA and compared among patients with sepsis (n = 75) and healthy volunteers (n = 15). (B) Serum IL-5 levels in patients with sepsis were subdivided based on their survival (left panel) or the need for mechanical ventilation (right panel). (C) Peripheral CD14+ neutrophils (left panel) or CD16+ (monocytes) from patients with sepsis were analyzed for IL-5Rα expression by flow cytometry. Unstained control is in black, IL-5Rα stained cells are in red. Histograms are representative plots of a subset of patients with sepsis. (D) The percentage of IL-5Rα expressing monocytes was assessed by flow cytometry and compared among patients with sepsis (n = 20) and nonseptic intensive care unit (ICU) control subjects (n = 5) (left panel) and was monitored over time in those patients with sepsis (right panel). Day 1 is at the time of admission. (A and D) Each symbol represents one individual subject. Error bars represent the means ± SEM. (E) THP-1 human monocytes were stimulated with LPS and IL-5Rα expression was assessed by flow cytometry. Unstained control is in black, IL-5Rα stained cells are in red. n = 3 experiments. (F) Serum levels of soluble IL-5Rα in patients with sepsis (n = 50) was measured by ELISA. Patients were subdivided based on the need for vasopressors (shock). Error bars represent the means ± SEM. Data were analyzed by Student t test; significance is defined as *P < 0.05; ***P < 0.001.