Figure 3.

Chlorine-induced acute lung injury increased bronchoalveolar lavage protein. Female mice were exposed to 45 ppm chlorine for 0 (filtered air control), 6, 12, or 24 (C57BL/10J only) hours and anesthetized, and bronchoalveolar lavage was performed with Ca²⁺, Mg²⁺-free PBS. Bronchoalveolar lavage protein increased sooner in the sensitive C57BLKS/J murine strain than in the resistant C57BL/10J murine strain. Lavage fluid was centrifuged, and total protein in cell-free supernatants was measured according to a bicinchoninic acid assay. Values represent means ± SE (n = 6 mice/strain/time). *Significantly different (P < 0.05) from strain-matched control mice, as determined by ANOVA with an all-pairwise multiple comparison procedure (the Holm-Sidak method). ^†Significantly different (P < 0.05) between the sensitive C57BLKS/J and resistant C57BL/10J murine strain at indicated times, as determined by ANOVA with an all-pairwise multiple comparison procedure (the Holm-Sidak method).