Figure 5.

(A) Quantitative real-time PCR showing the relative expression for syndecan-4 mRNA collected from bone marrow–derived macrophages (BMDMs) treated in vitro with RPMI media (control), two concentrations of the M1 agonist LPS (10 and 100 ng/ml), the M2a agonists IL-4/IL-13 (10 ng/ml), or the M2c agonist IL-10 for up to 48 hours (n = 4–13). (B) Quantitative real-time PCR showing the relative expression for syndecan-4 mRNA collected from alveolar macrophages treated with RPMI media (control) or LPS (100 ng/ml) for 4 hours (n = 4). (C and D) A representative histogram (C) and the geometric mean of syndecan-4 expression (D) on BMDMs measured with flow cytometry. Flow cytometry was performed 3 hours after the treatment of mouse BMDMs with RPMI or LPS (10 ng/ml) (n = 3). Values are the mean ± SEM. *Groups different from the control mice (P ≤ 0.05) using the Kruskal-Wallis test with Dunn's multiple comparison test (A) and the Mann-Whitney U test (B and D).